cloning and expression of mycobacterium tuberculosis esat-6 in prokaryotic system

Authors

e. asli

m. taghizadeh

a. rezaei mokaram

s.m. ebrahimi

a. zavaran hoseini

abstract

the identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. this study was designed for cloning and expression of esat-6 as a potent antigen of mycobacterium tuberculosis. selected gene (rv3875) was amplified by pcr and product was ligated into expressing plasmid vector pqe30 and recombinant pqe30-es plasmid was constructed. this hybrid vector was transformed in e. coli m15 and expressed in optimal condition. the expressed protein was analyzed on sds-page and confirmed by western blotting using specific antisera to esat-6. we successfully cloned and expressed esat-6 (his)6 from m. tuberculosis h37rv genome. as well as usage for serodiagnosis, this recombinant protein offers the potential development of other vaccine formats such as dna or subunit vaccines against tuberculosis.

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Journal title:
archives of razi institute

Publisher: razi vaccine & serum research institute (rvsri)

ISSN 0365-3439

volume 64

issue 1 2016

Keywords

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